ISO 19250-2010 PDF

Full title and description

ISO 19250:2010 — Water quality — Detection of Salmonella spp. (commonly referenced as St ISO 19250-2010). This International Standard specifies a laboratory method for the detection (presumptive or confirmed) of Salmonella species in water samples intended for drinking, recreational use and other environmental water monitoring purposes.

Abstract

ISO 19250:2010 defines procedures for recovering and identifying Salmonella spp. from water. It describes the general approach to sampling, enrichment, use of selective media and subsequent confirmation steps needed to report either presumptive or confirmed presence of Salmonella in water. The standard is intended to support routine monitoring, surveillance and outbreak investigation activities where detection of Salmonella in water is required.

General information

• Status: Published (confirmed in 2021)
• Publication date: 13 July 2010
• Publisher: ISO — International Organization for Standardization
• ICS / categories: 07.100.20 (Water quality)
• Edition / version: Edition 1 (2010)
• Number of pages: 23

Scope

This standard specifies a laboratory method for the detection of Salmonella spp. in water samples. It is applicable to water intended for drinking purposes and to recreational waters, and is suitable for routine monitoring, surveillance and for use during epidemiological or outbreak investigations (where additional media or confirmatory steps may be required). ISO 19250:2010 replaces ISO 6340:1995 and was prepared by ISO/TC 147/SC 4 (microbiological methods for water).

Key topics and requirements

• Principles for detection of Salmonella spp. in water matrices (presumptive and confirmed results).
• Recommendations for sample handling and preparation prior to analysis.
• Use of enrichment broths to enhance recovery of stressed or low-number Salmonella cells.
• Guidance on selective and differential media for isolation.
• Incubation and subculture steps leading to colony isolation.
• Approaches for presumptive identification (colony morphology, reactions on selective media).
• Confirmatory testing procedures (biochemical, serological or other appropriate confirmation methods).
• Reporting conventions distinguishing presumptive versus confirmed findings.
• Notes on limitations, quality control (use of controls and reference strains) and laboratory biosafety considerations.

Typical use and users

Typical users include water and environmental microbiology laboratories, public-health laboratories, municipal and utility water quality teams, regulatory agencies, contract testing laboratories, research institutions and organizations conducting outbreak investigations or surveillance for waterborne Salmonella. The standard supports regulatory compliance testing, routine monitoring and public-health risk assessments.

Related standards

Relevant related documents and series commonly used alongside ISO 19250 include the ISO 5667 series (water sampling), ISO 8199 (general guidance for microbiological examinations of water), ISO 9308 (microbiological methods for detection/enumeration of Escherichia coli and coliforms), ISO 11133 (performance and quality requirements for culture media) and ISO 16140 (method validation and verification for microbiological methods).

Keywords

Salmonella, water quality, detection, microbiology, drinking water, recreational water, enrichment, selective media, confirmation, ISO 19250

FAQ

Q: What is this standard?

A: ISO 19250:2010 is an International Standard that specifies laboratory methods for the detection of Salmonella species in water samples.

Q: What does it cover?

A: It covers principles and laboratory procedures for sample handling, enrichment, use of selective media, isolation, and confirmatory testing to report presumptive or confirmed Salmonella in water. It is applicable to drinking and recreational waters and to surveillance and outbreak investigations.

Q: Who typically uses it?

A: Water testing and public-health laboratories, regulatory bodies, utilities, environmental monitoring organizations, contract testing labs and researchers involved in waterborne pathogen monitoring and outbreak response.

Q: Is it current or superseded?

A: ISO 19250:2010 was published on 13 July 2010 and has been subject to systematic review; it was confirmed as current during review activity in 2021. It replaced the earlier ISO 6340:1995.

Q: Is it part of a series?

A: It belongs to the body of ISO standards on water quality and microbiological methods developed by ISO/TC 147/SC 4 and is typically used alongside other water-related standards such as the ISO 5667 sampling series, ISO 8199, ISO 9308 and standards addressing media and method validation.

Q: What are the key keywords?

A: Salmonella, water quality, detection, microbiology, drinking water, recreational water, enrichment, selective media, confirmation, ISO 19250.