ISO 21571-2005 PDF

Full title and description

Foodstuffs — Methods of analysis for the detection of genetically modified organisms and derived products — Nucleic acid extraction (ISO 21571:2005). The standard specifies general requirements and specific laboratory methods for the extraction, purification and quantification of nucleic acids (DNA) from food matrices to support downstream GMO detection methods.

Abstract

ISO 21571:2005 provides general requirements and one or more specific procedures for DNA extraction and purification from foodstuffs and related matrices, plus guidance on quantification to ensure suitability for nucleic-acid-based GMO analysis. Methods and examples are given in informative annexes; the standard was designed to work together with ISO 21569 (qualitative methods) and ISO 21570 (quantitative methods).

General information

• Status: Published; edition confirmed on review and remains current as of the last ISO confirmation.
• Publication date: February 2005 (Edition 1, 2005-02).
• Publisher: International Organization for Standardization (ISO).
• ICS / categories: 67.050 — Food analysis; methods for detection of genetically modified organisms.
• Edition / version: Edition 1 (2005); includes Amendment ISO 21571:2005/Amd 1:2013.
• Number of pages: 43 pages (main edition).

Scope

Specifies general requirements and specific laboratory procedures for extraction, purification and quantification of nucleic acids from foodstuffs and other related matrices (for example grains and feed) for the purpose of detecting genetically modified organisms and derived products. It is intended to provide suitable nucleic-acid preparations for use with ISO 21569 (qualitative) and ISO 21570 (quantitative) analytical methods.

Key topics and requirements

• General laboratory requirements for nucleic-acid extraction supporting GMO analysis (sample handling, controls, contamination prevention).
• Prescribed and example methods for DNA extraction and purification (described in annexes).
• Guidance on DNA quantification and assessment of extract quality for downstream PCR-based assays.
• Applicability to a range of food matrices, and notes on adapting methods to grains and animal feed.
• Includes an official amendment (Amd 1:2013) that updates or clarifies parts of the original text.

Typical use and users

Used by food testing laboratories, public health and regulatory laboratories, grain and feed testing facilities, contract testing organisations and research groups performing GMO detection and surveillance. The standard is used to obtain reproducible, inhibition-free DNA extracts suitable for qualitative and quantitative nucleic-acid-based assays.

Related standards

Designed to work with ISO 21569 (Methods of analysis for the detection of genetically modified organisms — qualitative methods) and ISO 21570 (quantitative methods). The technical work is under ISO/TC 34/SC 16 (Foodstuffs — Methods of analysis). The amendment ISO 21571:2005/Amd 1:2013 directly applies to this standard.

Keywords

ISO 21571, nucleic acid extraction, DNA extraction, GMO detection, foodstuffs, food analysis, PCR preparation, ISO/TC 34/SC 16.

FAQ

Q: What is this standard?

A: ISO 21571:2005 is an international standard specifying methods and requirements for extracting, purifying and quantifying nucleic acids from food matrices to support detection of genetically modified organisms.

Q: What does it cover?

A: It covers laboratory requirements, example extraction/purification procedures (annexes), and guidance on quantifying DNA and assessing extract suitability for downstream qualitative and quantitative GMO assays.

Q: Who typically uses it?

A: Food testing and regulatory laboratories, grain and feed testing facilities, contract testing labs and research organisations performing GMO analysis.

Q: Is it current or superseded?

A: The 2005 edition was reviewed and confirmed (last confirmation noted by ISO in 2020) and remains published; it also has one amendment published in March 2013 (ISO 21571:2005/Amd 1:2013). Users should check national or ISO catalogues for any later revisions beyond the stated confirmation date.

Q: Is it part of a series?

A: Yes — it forms part of a family of ISO methods for GMO detection and analysis, intended to be used with ISO 21569 (qualitative methods) and ISO 21570 (quantitative methods).

Q: What are the key keywords?

A: Nucleic acid extraction, DNA extraction, GMO detection, foodstuffs, PCR, analytical methods, ISO 21571.