ASTM D8025-23 PDF

Full title and description

Standard Test Method for Determination of Select Pesticides in Water by Multiple Reaction Monitoring Liquid Chromatography Tandem Mass Spectrometry (ASTM D8025-23). This method defines sample preparation (20% methanol, filtration) and analysis by liquid chromatography with electrospray ionization tandem mass spectrometry operated in multiple reaction monitoring (MRM) mode to qualitatively and quantitatively determine a specified suite of pesticides in water matrices, primarily developed for agricultural run-off studies.

Abstract

ASTM D8025-23 specifies a rapid, robust LC–MS/MS MRM procedure for analysis of selected pesticides in water. Samples are prepared by dilution into 20% methanol and filtration, then analyzed by LC coupled to ESI-tandem mass spectrometry using MRM transitions for target analytes. The standard includes reporting ranges, reporting-limit checks (RLCS) for batch QC, and single-laboratory validation data; it was developed for agricultural run-off monitoring rather than ultra-trace drinking-water analysis.

General information

• Status: Active.
• Publication date: April 15, 2023 (issued as D8025-23).
• Publisher: ASTM International.
• ICS / categories: 13.060.50 (Examination of water for chemical substances).
• Edition / version: D8025-23 (current revision; replaces earlier D8025 editions).
• Number of pages: 24 pages.

Key bibliographic and status details are recorded by ASTM and standard distributors.

Scope

This test method covers analysis of selected pesticides in water by filtration followed by LC/ESI–MS/MS using MRM. It describes sample preparation (final matrix ~80% water/20% methanol for analysis), calibration/reporting limits, and required batch quality checks (including a Reporting Limit Check Sample, RLCS). The procedure was validated in reagent and surface waters by single-laboratory validation; a full collaborative study per Practice D2777 was not completed prior to publication. The method is intended for agricultural run-off monitoring and may be modified for lower-level drinking-water analyses if additional validation (e.g., MDL studies) is performed.

Key topics and requirements

• Technique: Liquid chromatography with electrospray ionization tandem mass spectrometry (LC–ESI–MS/MS) using multiple reaction monitoring (MRM).
• Sample preparation: Dilution into 20% methanol and filtration (final analysis matrix ≈80% water/20% methanol).
• Analytes: Suite of selected pesticides (examples include atrazine, simazine, metolachlor, acetochlor, imidacloprid, clothianidin, fipronil, diazinon, chlorpyrifos and others listed in the standard).
• Quality control: Calibration levels, reporting limits based on Level 1 calibration, Reporting Limit Check Sample (RLCS) analyzed each batch; MDL study not required for this publication level but may be needed if adapted for lower reporting levels.
• Validation: Single-laboratory validation data provided for reagent water and surface waters; collaborative study per Practice D2777 was not completed at time of publication.

These technical requirements and analyte listings are specified in the published D8025-23 document.

Typical use and users

Environmental testing laboratories, water-quality research groups, agricultural run-off monitoring programs, regulatory and compliance laboratories, and instrument manufacturers developing LC–MS/MS methods will use this standard. Its primary application is routine monitoring and investigative studies of pesticide occurrence in surface and run-off waters rather than ultra-trace drinking-water compliance without further method adaptation and validation.

Related standards

Related or referenced documents include earlier editions of ASTM D8025 (for example D8025-16), Practice D2777 (collaborative study practice referenced for interlaboratory validation), and other water-examination and analytical-method standards within ASTM D19 committee publications addressing chemical analysis of water. Committee D19.06 developed this method.

Keywords

pesticides, water analysis, LC–MS/MS, liquid chromatography, tandem mass spectrometry, MRM, electrospray ionization, reporting limit, RLCS, agricultural run-off, atrazine, imidacloprid, fipronil, metolachlor.

FAQ

Q: What is this standard?

A: ASTM D8025-23 is a Standard Test Method that specifies an LC–ESI–MS/MS multiple reaction monitoring procedure for determining selected pesticides in water matrices; it was published as the 2023 revision.

Q: What does it cover?

A: It covers sample preparation (20% methanol dilution and filtration), instrument method using MRM on LC–MS/MS, calibration and reporting limits, and batch QC including a Reporting Limit Check Sample (RLCS). The method was developed for agricultural run-off and provides single-laboratory validation results.

Q: Who typically uses it?

A: Environmental and commercial analytical laboratories, water-quality researchers, regulatory bodies monitoring pesticide impacts on surface waters, and laboratories supporting agricultural run-off studies.

Q: Is it current or superseded?

A: As of its publication, D8025-23 is the current active revision (published April 15, 2023) and supersedes prior editions of D8025. Users should check ASTM records for any subsequent revisions.

Q: Is it part of a series?

A: Yes — it is part of ASTM's suite of water examination standards maintained by Committee D19 (subcommittee D19.06) and is published within the ASTM Volume covering water standards; it references other ASTM practices such as Practice D2777 for collaborative study procedures.

Q: What are the key keywords?

A: Key keywords include LC–MS/MS, MRM, pesticides, water analysis, electrospray ionization, reporting limit, RLCS, and specific pesticide names such as atrazine, imidacloprid, fipronil, and metolachlor.